The purpose of the toxicity testing program is to evaluate potential toxicity of agriculture drain water as it is conveyed through the SLD to Mud Slough and removed from Salt Slough. The toxicity testing program includes a combination of laboratory methods using standard protocols and field methods. At the same time, water samples will be collected for selenium and sulfate analysis. In addition, at the end of the test, control and exposed algae will be analyzed for selenium bioaccumulation.